Protein Modification Screening Tool
(ProMoST)
Program to calculate accurate MWT and pI values from proteins
Accession Numbers:
List of accession numbers goes here.
File of Accession numbers to upload:
pK values
Basic Amino Acids
AA
Primary
N-Terminal
C-Terminal
K
R
H
Acidic Amino Acids
AA
Primary
N-Terminal
C-Terminal
D
E
C
Y
Terminal Amino Acids
Amino Acid
N-term
C-Term
G
A
S
P
V
T
C
I
L
N
D
Q
K
E
M
H
F
R
Y
W
Protein Modifications
Terminal Ends Blocked
N Terminus
C Terminus
Both ends
Deamidation
Asparagine(N)
1
1 to 2
1 to 3
1 to 4
1 to 5
1 to 10
Glutamine(Q)
1
1 to 2
1 to 3
1 to 4
1 to 5
1 to 10
Phosphorylation
Phosphoserine/threonine(S/T)
1
1 to 2
1 to 3
1 to 4
1 to 5
1 to 10
pKa1
pKa2
Phosphotyrosine(Y)
1
1 to 2
1 to 3
1 to 4
1 to 5
1 to 10
pKa1
pKa2
Additional Modifications
Modification 1 Name
Charge
Positive
Negative
pKa1
pKa2
Modification 2 Name
Charge
Positive
Negative
pKa1
pKa2
Modification 3 Name
Charge
Positive
Negative
pKa1
pKa2
Display options:
Input
Sequence Title Lines
Amino Acid Sequence
Amino Acid Composition
Output locations:
Display on Screen
Save to Tab Delim Text File
Plot gel image
Low pH
High pH
pH step
Low MWT (kDa)
High MWT (kDa)
MWT step (kDa)
Output options:
Detailed header
Column headings
Input Acc. Numbers
Acc. Numbers
Sequence description
Monoisotopic mass
Average isotopic mass
pI